Introduction:

  • An ISO 9001:2015 lab established under Tata Trusts funding (Grant No. – Health-BSDT-20151007) for immunological and molecular marker analysis of cancer patients.
  • More than 2000 samples have been assayed for these markers under four clinical projects on breast and oral cavity cancers (Funded by Tata Trusts – 2015 to 2021) and additional 4500 samples stored in -80 °C for future analysis.
  • Eleven Ayurvedic formulations studied in vitro by phytochemical (Total Phenol, Total Tannin, Total Flavonoids) and anti-oxidant assays (DPPH, Phospho-molybdate).
  • Recently developed an Animal Tissue Culture Lab for cell-line maintenance and cytotoxicity MTT assay
  • A Zebrafish Animal Experimentation Lab developed for FET assay, Artemia preparation, breeding and staining of fish under the able guidance of Emeritus Prof. Dr Nishigandha Naik.
  • Preliminary FET assay and MTT assay on A375, MCF-7, MCF-10A, and MDA-MB-231 cell lines have been performed for Ayurvedic formulations like Arogyavardhini Vati, Khadhirarishta and Raupya Suvarna Sutashekhar Rasa.

Facilities:

  • Molecular analysis using DNA-RNA Isolation, PCR and Gel Electrophoresis
  • Blood processing for ELISA readings of cytokines (IL1, IL6, IL8, IL10) and redox markers (SOD, Catalase, Glutathione).
  • ELISA based phytochemical and antioxidant assays
  • FET assay, cardiotoxicity, fin-regeneration, cartilage and bone growth studies in Zebra fish model
  • MTT cytotoxicity assay on A375, MCF-7, MCF-10A, and MDA-MB-231 cell lines.

Current projects:

  • Estimation of thioethers in urine samples of addicted and non-addicted cancer and non-cancer patients.
  • Sample collection and Serum separation of various blood samples for different proteomics studies.